Comparison between V-Tubes and BD Vacutainer Tubes
for Use in Laboratory Tests
Eun Jeong Won, M.D.1, Myung-Geun Shin, M.D.1, Min-Joong Jang, M.D.1, Duck Cho, M.D.1, Seung-Jung Kee, M.D.1, Soo-Hyun Kim, M.D.1, Jong Hee Shin, M.D.1, Yonggwan Won, M.D.2, Dong-Wook Ryang, M.D.1, Soon-Pal Suh, M.D.1
Department of Laboratory Medicine1, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju; School of Electronics and Computer Engineering2, College of Engineering, Chonnam National University, Gwangju, Korea
- Background: Vacuum tubes are widely used in clinical laboratories for routine tests. We compared a newly developed V-tube (AB Medical, Korea)and BD tubes (BD, USA) in common clinical assays, i.e., hematological, chemical, and immunological tests.
- Methods: In total, 100 volunteers comprising 79 patients and 21 healthy volunteers were recruited and peripheral blood samples were collected with 2 brands of EDTA tubes and serum-separating tubes (SSTs). EDTA-tube samples were evaluated using 16 routine hematological tests. The SST samples were analyzed for 32 routine chemical parameters and 3 thyroid hormones. The results were statistically analyzed using the paired ttest and Bland-Altman plot. In addition, the stability of each analyte in 2 brands of vacutainers was evaluated. The results of the hematological tests at t=0 hr were compared with those at t=72±2 hr, and the results of the chemical parameters and thyroid hormones at t=0 hr were compared with those at t=72±2 hr and t=168±2 hr for each tube.
- Results: Paired t-test analysis revealed that the test results of 16 routine hematological parameters, 32 routine chemical parameters, and 3 thyroid hormones showed clinically allowable differences between the 2 brands of vacuum tubes (t =0 hr). The results obtained when using V-tubes showed a statistically significant correlation with those obtained when using BD tubes. The stability of each analyte was similar in both vacuum tubes. Except for 10 parameters (white blood cell count, mean corpuscular volume, basophils [ %], mean corpuscular hemoglobin concentration, monocytes [ %], phospholipid, sodium, potassium, chloride, and free T4), all parameters showed significant but clinically allowable differences with regard to storage duration.
- Conclusions: The newly developed V-tube vacutainers provide a suitable alternative to BD tubes in common clinical laboratories.
Key Words:
V-tube, BD tube, Comparison
